PTXL: PowerTome

The PowerTome is the only fully upgradeable ultramicrotome, with the ability to take a PTXL, the routine workhorse, and build it up to the PTPCZ research level system with computer control and HD video options. The customer has the choice of upgrading to a PC-based controller with or without the high-definition video package.

The PTXL is controlled via a digital tactile controller, eminently suitable for routine work. The PowerTome is capable of accommodating glass knives up to 12mm wide, triangular tungsten carbide knives, and any commercial brand of diamond knife.

The PTXL can be upgraded to the PTPC, PTPCZ, and PT3D for use with a computer-based controller with HD video options. Various add-on components for array tomography and cryo applications are also available.

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PTPCZ : PowerTome with High Definition Video & Real-Time Display

The PTPCZ features a high-definition video package where the sectioning process is displayed in real-time at the center of the computer monitor. Equipped with a computer control interface, the PTPCZ enables the viewing and recording of the sectioning process in full HD for future viewing and use.

The all-in-one computer controller features a slim design 21.5” touch screen control monitor, including a wireless mouse & keyboard, interconnect cables & accessories. All control functions can be activated by touching the control monitor screen, or by mouse while maintaining the use of the tactile digital controller. The color monitor displays a bright and user-friendly control interface with intuitive control icons logically arranged around the video display.

The PTPCZ can be upgraded to the PT3D, featuring 1 mm of uninterrupted sectioning. Various add-on components for array tomography and cryo applications are also available.

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PT3D: PowerTome with 1mm Advance for Serial Sectioning

The PT3D features a full 1 mm of advance, permitting uninterrupted, ultra-thin, sectioning to a depth of one millimeter. This unique advantage is especially beneficial for 3D reconstruction work, array tomography, and any situation where high amounts of precision specimen trimming are involved.

The all-in-one computer controller features a slim design 21.5” touch screen control monitor, including a wireless mouse & keyboard, interconnect cables & accessories. All control functions can be activated by touching the control monitor screen, or by mouse while maintaining the use of the tactile digital controller. The color monitor displays a bright and user-friendly control interface with intuitive control icons logically arranged around the video display.

Various add-on components for array tomography are also available.

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ATUMtome: Automated Tape Collecting Ultramicrotome

The expansion and advancement of microscopical applications has led to exciting new developments, not least in the field of 3D Array Tomography and Volume Imaging. 

At Harvard University, Professor Jeff Lichtman, PhD, MD, and his team designed an instrument (ATUM) capable of collecting very large sets of serial sections (30,000+) in an unattended and computer-controlled operation, to help reconstruct the brain’s neural pathways — a precursor to one day mapping the entire human brain. The ATUM is connected to and controlled by RMC Boeckeler’s PowerTome ultramicrotome, to form the ATUMtome (Automatic Tape Collecting Ultramicrotome). 

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ASH2: Advanced Substrate Holder Attachment for Serial Sectioning

For Fast, Reliable Serial Section Collection For 3D Tomography.

Advanced 3D reconstruction and tomography are growing in use in today’s EM world. For small volumes, where tens to hundreds of sections are required, the Advanced Substrate Holder (ASH2) is favored for ease of use and speed.

The ASH2 is a multi-axis manipulation tool designed to precisely position substrates for the collection of ribbons of serial sections for array tomography. Standard room temperature knives and jumbo diamond knives for larger substrates can be used. Collected sections are subsequently imaged in the SEM or LM.

The ASH2 quickly mounts onto any current ultramicrotome, instantly converting it into an instrument capable of sectioning and collecting hundreds of sections. This newly designed Advanced Substrate Holder (ASH2) weighs less than 0.5kg and can be mounted directly onto modern ultramicrotomes without modification or the use of additional fixtures.

For larger volume work, please see our ATUMtome system, which is capable of effortlessly collecting thousands of sections.

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LN Ultra: Cryo Attachment for PowerTome

Rapid freezing and Freeze Substitution are one method of making cellular structure and organization appear closer to the natural state but this process can limit other avenues of investigation, such as immunohistochemical applications.

Dehydration of a sample, necessary for routine electron microscopy of biological tissue, can be a major source of artifact and in many cases inhibits antibody-antigen reactions essential for immunolabelling/gold labeling studies. If a method could be found to preserve the water content in the cell during the process, this would greatly increase the scope of investigations, and the work of Professor K.T. Tokuyasu provided this breakthrough.

Today, cryosectioning and labeling of tissues and cell cultures by the ‘Tokuyasu Technique’ is a routine application in many laboratories.

Cryo ultramicrotomy sectioning of both biological and industrial materials can be achieved with the LN Ultra, a liquid nitrogen-cooled cryo-chamber attachment for the PowerTome series of ultramicrotomes. Mounted onto the PowerTome, the LN Ultra provides optimal thermal stability necessary for producing cryosections.

Capable of holding temperatures from +40°C to -185°C, the stable environment of the LN Ultra is perfect for sectioning and planning a variety of samples. These may include rubber and soft polymers, or biological cells and tissues; used for example, for immunolabelling using the Tokuyasu technique or CEMOVIS (Cryo-Electron Microscopy of Vitreous Ice Sections).

Liquid Nitrogen consumption is very low, allowing for a size efficient 15.5-liter dewar, capable of providing a stable sectioning environment for up to nine hours, depending on your technique and application, thus saving your laboratory LN2 costs over less efficient systems.

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MR2: Manual Rotary Microtome

Traditional, Rugged Reliability.

For routine work, the MR2 and its motorized advance partner, the MR3 are available, both frequently being used for histology and materials preparation. Both rotary microtomes feature a rapid trim function, positive mechanical lock on the hand wheel, specimen orientation and rotation, plus a digital section counter display on the front panel.

• Heavy base casting for stable sectioning
• Vertical cutting stroke with solid cross roller bearing specimen carriage
• Control panel with Digital Section Counter Display and easy-to-clean membrane switches
• Electronic feed with fast return
• Separate trim control function with footswitch
• Preset trim setting for fast block trimming
• Removable waste tray
• Wide range of accessories available for Histology, Materials, and Forensic applications
• Available with High/Low Profile Disposable Steel Blade Holder and Specimen Holder (choice of Cassette Clamp or Fixed Jaw Vise Clamp)
• MR2 supplied with single footswitch control for trim function

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MR3: Motorized Rotary Microtome

Universal, Heavy Duty Microtome For Motorized Operation

For routine work, the MR3 is frequently used for histology and materials preparation. The rotary microtome features a rapid trim function, a positive mechanical lock on the hand wheel, specimen orientation and rotation, plus a digital section counter display on the front panel.

• Heavy base casting for stable sectioning
• Vertical cutting stroke with solid cross roller bearing specimen carriage
• Control panel with Digital Section Counter Display and easy-to-clean membrane switches
• Electronic feed with fast return
• Separate trim control function with footswitch
• Preset trim setting for fast block trimming
• Removable waste tray
• Wide range of accessories available for Histology, Materials, and Forensic applications
• Available with High/Low Profile Disposable Steel Blade Holder and Specimen Holder (choice of Cassette Clamp or Fixed Jaw Vise Clamp)

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MT990: Rotary Microtome

For automated sectioning, the MT990 rotary microtome features a motorized, programmable, automatic trim providing a reproducible, consistent cutting speed. With an accelerated return stroke and adjustable cutting zone, the MT990 effortlessly cuts paraffin, plastic, and hard specimens. The advantages of the motor drive can also be used to reduce the strain of repetitive, high output histology sectioning. Included with a separate controller for hands-off operation of the motorized cutting stroke, the MT990 may also be used manually. Features include:

• Tactile control unit: Digital feed totalizer, selectable for either total feed in microns or total number of sections
• Cutting mode control with; continuous cut, single stroke, intermittent cut
• 4-channel memory for storing section thickness/cutting speed combinations
• Fast coarse feed advance/reverse control
• Step advance for electronic trimming
• Foot Pedal for hands-off control of cut and trim functions
• Optional Binocular Microscope Accessory with Backlighting Accessory
• Optional cryo attachment (CR1000) permits sectioning from +40°C to -160°C

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MT990 with CR1000: Rotary Microtome with Cryo Attachment

Ideal tool for cryosectioning polymers, rubbers, and films for optical and FTIR transmission microscopy, as well as cryo-planing sample surfaces for AFM and SEM. Diamond and glass knives can be placed in the rotatable knife holder.

The CR1000 is equipped with independent temperature controls for the knife and specimen.

In conjunction with the CR1000, the MT990 becomes the world’s only cryo microtome for light microscopy, capable of preparing large areas of rubber, polymer, and biological samples.

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EM Pro: Automated Tissue Processor

For labs handling high volumes of tissue specimen embedding, the EM Pro is the ultimate productivity tool. Simply set your desired parameters—time, agitation, and temperature—and let the EM Pro’s advance automated robotic system precisely transfer specimen baskets from one vial to the next. Its sealed system protects your samples throughout the process, ensuring consistency and reliability.

Achieve consistent, reproducible, and hassle-free embedding with the EM Pro!

• Easy touchscreen programming: Integrated 7-inch HD touchscreen with 4 memory presets and the ability to store over 100 unique programs
• Sealed system with active exhaust: Vial caps prevent the escaping and gradual depletion of chemical volume. Operating outside a fume hood is possible using the included active fan exhaust extension system.
• Economical! Only small volumes of reagents are needed for each vial.
• Programmable agitation: Set the frequency which best meets your sample requirements.
• Complete with two specimen holder systems:
  Choose between the traditional stack rings or multi-compartment baskets.
  Stack rings are ideal for larger samples or groups, while compartmentalized baskets enable simultaneous processing of different specimen types.
• Small tabletop footprint
• Quick release exhaust hose

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GKM2: Glass Knife Maker

The harder embedding media needed to support the tissue during the sectioning process and subsequent observation in the electron microscope necessitates a blade that is precise and robust enough to cut the very thin sections on the ultramicrotomes. Freshly broken glass with its super sharp edge provides an excellent solution for cutting the ultra-thin sections necessary.

In addition, the glass knife is preferred for teaching, trimming, and for those unknown samples which may have inclusions that can instantly damage your diamond edge.

The GKM2 glass knife maker produces the most reproducible straight-edged glass knives available. With its balanced break methodology coupled with a pressure detector readout for precise reproducibility of breaking pressure, the GKM2 is the favored knifemaker for many laboratories. The GKM2 is also the only glass knife maker in the market capable of producing up to 12mm thick glass knives.

The pressure sensor and digital display enable the operator to produce accurate knives with each controlled break. Precision micrometers accurately position glass squares for the final break into knives.

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Anti-Vibration Table

With advanced air-activated pistons for both vertical and horizontal vibration isolation. Suitable for all RMC Microtomes, ultramicrotomes, and cryosectioning systems.

Highly recommended for ultramicrotomes and cryosectioning systems, as well as microtomes.

Equipped with a heavy stainless steel table top, 750mm x 1200mm x 50mm thick (29.5” x 47.2” x 2”), a pneumatic vibration isolation system, leveling feet, and four retractable casters.

Includes both front and rear rails to support two 360mm (14 inches) wide white shelves which are included. These shelves are isolated from the tabletop. A bottom shelf is also included for the support of power supplies and other equipment.

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